167 research outputs found
Perturbed cholesterol and vesicular trafficking associated with dengue blocking in Wolbachia-infected Aedes aegypti cells
Wolbachia are intracellular maternally inherited bacteria that can spread through insect populations and block virus transmission by mosquitoes, providing an important approach to dengue control. To better understand the mechanisms of virus inhibition, we here perform proteomic quantification of the effects of Wolbachia in Aedes aegypti mosquito cells and midgut. Perturbations are observed in vesicular trafficking, lipid metabolism and in the endoplasmic reticulum that could impact viral entry and replication. Wolbachia-infected cells display a differential cholesterol profile, including elevated levels of esterified cholesterol, that is consistent with perturbed intracellular cholesterol trafficking. Cyclodextrins have been shown to reverse lipid accumulation defects in cells with disrupted cholesterol homeostasis. Treatment of Wolbachia-infected Ae. aegypti cells with 2-hydroxypropyl-β-cyclodextrin restores dengue replication in Wolbachia-carrying cells, suggesting dengue is inhibited in Wolbachia-infected cells by localised cholesterol accumulation. These results demonstrate parallels between the cellular Wolbachia viral inhibition phenotype and lipid storage genetic disorders
Ultraconserved element (UCE) probe set design: Base genome and initial design parameters critical for optimization
This work is licensed under a Creative Commons Attribution 4.0 International License.Targeted capture and enrichment approaches have proven effective for phylogenetic study. Ultraconserved elements (UCEs) in particular have exhibited great utility for phylogenomic analyses, with the software package phyluce being among the most utilized pipelines for UCE phylogenomics, including probe design. Despite the success of UCEs, it is becoming increasing apparent that diverse lineages require probe sets tailored to focal taxa in order to improve locus recovery. However, factors affecting probe design and methods for optimizing probe sets to focal taxa remain underexplored. Here, we use newly available beetle (Coleoptera) genomic resources to investigate factors affecting UCE probe set design using phyluce. In particular, we explore the effects of stringency during initial design steps, as well as base genome choice on resulting probe sets and locus recovery. We found that both base genome choice and initial bait design stringency parameters greatly alter the number of resultant probes included in final probe sets and strongly affect the number of loci detected and recovered during in silico testing of these probe sets. In addition, we identify attributes of base genomes that correlated with high performance in probe design. Ultimately, we provide a recommended workflow for using Phyluce to design an optimized UCE probe set that will work across a targeted lineage, and use our findings to develop a new, open‐source UCE probe set for beetles of the suborder Adephaga.NIH IRACDA postdoctoral fellowship (5K12GM063651)Harold E. and Leona M. Rice Endowment Fund at Oregon State Universit
Motion of spiral waves in the Complex Ginzburg-Landau equation
Solutions of the general cubic complex Ginzburg-Landau equation comprising
multiple spiral waves are considered. For parameters close to the vortex limit,
and for a system of spiral waves with well-separated centres, laws of motion of
the centres are found which vary depending on the order of magnitude of the
separation of the centres. In particular, the direction of the interaction
changes from along the line of centres to perpendicular to the line of centres
as the separation increases, with the strength of the interaction algebraic at
small separations and exponentially small at large separations. The
corresponding asymptotic wavenumber and frequency are determined. These depend
on the positions of the centres of the spirals, and so evolve slowly as the
spirals move
Helicoidal instability of a scroll vortex in three-dimensional reaction-diffusion systems
We study the dynamics of scroll vortices in excitable reaction-diffusion
systems analytically and numerically. We demonstrate that intrinsic
three-dimensional instability of a straight scroll leads to the formation of
helicoidal structures. This behavior originates from the competition between
the scroll curvature and unstable core dynamics. We show that the obtained
instability persists even beyond the meander core instability of
two-dimensional spiral wave.Comment: 4 pages, 5 figures, revte
Scroll waves in isotropic excitable media : linear instabilities, bifurcations and restabilized states
Scroll waves are three-dimensional analogs of spiral waves. The linear
stability spectrum of untwisted and twisted scroll waves is computed for a
two-variable reaction-diffusion model of an excitable medium. Different bands
of modes are seen to be unstable in different regions of parameter space. The
corresponding bifurcations and bifurcated states are characterized by
performing direct numerical simulations. In addition, computations of the
adjoint linear stability operator eigenmodes are also performed and serve to
obtain a number of matrix elements characterizing the long-wavelength
deformations of scroll waves.Comment: 30 pages 16 figures, submitted to Phys. Rev.
Recommended from our members
Genome-wide variation and transcriptional changes in diverse developmental processes underlie the rapid evolution of seasonal adaptation
Many organisms enter a dormant state in their life cycle to deal with predictable changes in environments over the course of a year. The timing of dormancy is therefore a key seasonal adaptation, and it evolves rapidly with changing environments. We tested the hypothesis that differences in the timing of seasonal activity are driven by differences in the rate of development during diapause in Rhagoletis pomonella, a fly specialized to feed on fruits of seasonally limited host plants. Transcriptomes from the central nervous system across a time series during diapause show consistent and progressive changes in transcripts participating in diverse developmental processes, despite a lack of gross morphological change. Moreover, population genomic analyses suggested that many genes of small effect enriched in developmental functional categories underlie variation in dormancy timing and overlap with gene sets associated with development rate in Drosophila melanogaster. Our transcriptional data also suggested that a recent evolutionary shift from a seasonally late to a seasonally early host plant drove more rapid development during diapause in the early fly population. Moreover, genetic variants that diverged during the evolutionary shift were also enriched in putative cis regulatory regions of genes differentially expressed during diapause development. Overall, our data suggest polygenic variation in the rate of developmental progression during diapause contributes to the evolution of seasonality in R. pomonella. We further discuss patterns that suggest hourglass-like developmental divergence early and late in diapause development and an important role for hub genes in the evolution of transcriptional divergence
Characterisation of the enzyme transport path between shipworms and their bacterial symbionts.
BACKGROUND: Shipworms are marine xylophagus bivalve molluscs, which can live on a diet solely of wood due to their ability to produce plant cell wall-degrading enzymes. Bacterial carbohydrate-active enzymes (CAZymes), synthesised by endosymbionts living in specialised shipworm cells called bacteriocytes and located in the animal's gills, play an important role in wood digestion in shipworms. However, the main site of lignocellulose digestion within these wood-boring molluscs, which contains both endogenous lignocellulolytic enzymes and prokaryotic enzymes, is the caecum, and the mechanism by which bacterial enzymes reach the distant caecum lumen has remained so far mysterious. Here, we provide a characterisation of the path through which bacterial CAZymes produced in the gills of the shipworm Lyrodus pedicellatus reach the distant caecum to contribute to the digestion of wood. RESULTS: Through a combination of transcriptomics, proteomics, X-ray microtomography, electron microscopy studies and in vitro biochemical characterisation, we show that wood-digesting enzymes produced by symbiotic bacteria are localised not only in the gills, but also in the lumen of the food groove, a stream of mucus secreted by gill cells that carries food particles trapped by filter feeding to the mouth. Bacterial CAZymes are also present in the crystalline style and in the caecum of their shipworm host, suggesting a unique pathway by which enzymes involved in a symbiotic interaction are transported to their site of action. Finally, we characterise in vitro four new bacterial glycosyl hydrolases and a lytic polysaccharide monooxygenase identified in our transcriptomic and proteomic analyses as some of the major bacterial enzymes involved in this unusual biological system. CONCLUSION: Based on our data, we propose that bacteria and their enzymes are transported from the gills along the food groove to the shipworm's mouth and digestive tract, where they aid in wood digestion
- …